种属

人

别称

UCH-1; UCH1

组织来源

脊柱；骶骨

疾病

脊索瘤

传代比例/细胞消化

1:2传代 ，消化2-3分钟

完全培养基配置

80% mixture of Iscove's MDM and RPMI 1640 (at 4:1)；10%胎牛血清；1%双抗

背景描述

U-CH1 is the first human chordoma cell line. It exhibits chordoma-like characteristics and has

molecular, genetic, and morphological features typical of chordoma. This cell line was established

from a local recurrence of a sacrococcygeal chordoma after radiotherapy 4 years after initial surgery. Chordoma is a rare slow-growing tumor type, and U-CH1 is a relatively slow-growing cell line. U-CH1 has a heterogeneous morphology consisting of physaliferous cells with mcinous intercellular

substance, which represent typical chordoma features. The cells overexpress the transcription factor T (Brachyury) that is the most specific marker for chordoma. This cell line was accessioned with the

support of the Chordoma Foundation, a non-profit organization working to improve the lives of

chordoma patients by accelerating research to develop effective treatments for the chordoma disease.

形态

间充质细胞样

生长特征

贴壁生长

STR

Amelogenin X,Y CSF1PO 10,11 D1S1656 16,16.3 D2S441 12,14 D2S1338 17,18 D3S1358 15 D5S818 11,12 D7S820 9,12 D8S1179 10,15 D10S1248 15 D12S391 17,22 D13S317 11,13 D16S539 12,13

D18S51 15 D19S433 14 D21S11 28,29 D22S1045 16 FGA 20,21 Penta D 11 Penta E 7,10 TH01 7 TPOX 8,11 vWA 17

倍增时间

每周 2 至 3 次

培养条件

气相：空气 ，95% ；二氧化碳 ，5%。 温度：37摄氏度 ，培养箱湿度为70%-80%。

冻存条件

冻存液：90%FBS ，DMSO 10%,

或使用非程序冻存液：官网货号JY-H040

保藏机构

ATCC; CRL-3217

产品使用

仅限于科学研究 ，不可作为动物或人类疾病的治疗产品使用。